Myagri is committed to providing products and services that meet customer requirements and applicable standards and specifications. Each employee is committed to this goal.
The company's Quality Policy is to continually improve our product and performance by supplying goods and services of a quality standard that shall consistently meet the requirements of our customers.
We will ensure a high level of customer satisfaction to maintain long term commitments, productive customer relationships, and to provide a positive work environment.
All our personnel are responsible for the attainment and maintenance of this quality standard.
IN SUMMARY
MYAGRI is committed:-
· to delivering products as per specifications committed to the clients in a timely manner;
· to continuously improving quality through Research and Development and technical advancement; and
· to maintaining awareness and compliance of the quality management system (QMS) by all MYAGRI’s employees, sub-contractors, vendors and collaborators
WET SIEVING AND DECANTING TECHNIQUE-Gerdemann and Nicolson, 1963.
The wet sieving and decanting is one of the popular technique when compare to other techniques. This technique is used for sieving the coarse particles of the soil and retaining AMF spores and organic particles on sieves of different sizes.10 g of soil was mixed with 100ml of water in the 500 ml conical flask. The soil mixture was agitated vigorously to free the AMF spores from soil and allowed to settle and the supernatant was decanted through standard sieves. By using a dissecting microscope, spores were picked by means of pipette or needle.
Arbuscular mycorrhizal fungi (AMF) spores are normally isolated by wet sieving and decanting method. AMF spores were isolated by capillary rise method. The rise in the capillary is due to the result of surface tension of liquid.
Gerdemann and Nicolson, 1963 developed wet sieving and decanting method to isolate different size of Arbuscular mycorrhizal fungi (AMF) spores.
16 Wet-sieving and Decanting Techniques for the Extraction of Spores of Vesicular-arbuscular Fungi
December 1992
DOI: 10.1016/S0580-9517(08)70099-0
Authors : Giovanni Pacioni 35.19Universitàdegli Studi dell'Aquila
Pacioni, Giovanni. (1992). 16 Wet-sieving and Decanting Techniques for the Extraction of Spores of Vesicular-arbuscular Fungi. Methods in Microbiology. 24. 317-322. 10.1016/S0580-9517(08)70099-0.
Spores of arbuscular fungi are isolated by us using the wet sieving and decanting method described by Gerdemann and Nicolson (1963). The procedure used is as follows:
Step 1. 100 g of air-dried root-rhizosphere soil mixture is placed into a glass container with 1000 ml of tap water. When the mixture contains rough soil, the glass with water and the mixture is kept in a refrigerator at 4oC for at least 12 h.
Step 2. The root-soil mixture is vigorously mixed with a glass rod for 30 sec.
Step 3. After 10-second pause enabling to settle heavier particles and organic material, the remaining soil-root-hyphae-spore suspension is slowly poured through a set of two sieves. The sieves used are those with pores of diameters of 0.5 (the top one) and 0.045 mm. Most spores retain on the 0.045 mm sieve. The top sieve isolates large sporocarps and spores associated with roots.
Step 4. The extracts are washed away from the sieves to Petri dishes of a diameter of 10 cm.
Step. 5. Using a dissecting microscope, spores, aggregates, and sporocarps are picked by means of pipette or needle.
